Product Name
Bac-to-Bac Expression Kit
Link To
Expression Related Kit
Expression System
Insect Expression System
Category
Protein expression kit
Application
Protein expression in the baculovirus system
Recommend Cell Line
Sf9, Sf21, and High Five
Subclone Construction Method
Restriction Enzyme ⁄ MCS
Recombination Method
Tn7 transposition
Industry Terms
Not Available to Industry
Contents
1. pFastBac1 vector (10 µg), store at -20°C
2. pFastBac 1-Gus control vector (4 ng), store at -20°C
3. DH10Bac Chemically Competent E. coli (1 kit with 5 × 0.1 ml), store at -80°C
4. Transfection Reagent (1 ml), store at 4°C, do not freeze
Description
The Bac-to-Bac baculovirus expression system enables the efficient production of recombinant baculovirus for expression testing in insect cells. The system relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
