Lactococcus Lactis Surface Display Service

Lactococcus lactis is recognized as food-grade Gram-positive bacteria, which is widely used in biotechnology, food and medicine. In order to further develop the function of Lactococcus lactis expression system, Creative BioMart has developed our own Lactococcus lactis surface display system by means of genetic engineering. The system can express and display a variety of foreign proteins on the surface of Lactococcus lactis, so that the biological functions of proteins can be displayed.

We will provide customers with a one-stop service from expression scheme design to protein display. We offer both constitutive and inductive surface display systems for customers to choose from. If you are interested in our service or have any questions, please feel free to contact us.

When Should You Choose Lactococcus lactis Surface Display Service

The Lactococcus lactis Surface Display Service is suitable for customers who want to study

• Presentation of epitopes for vaccine development and drug delivery
• Pharmaceutical active peptide substance delivery for oral administration
• Display of immobilized enzymes for chemical production
• Whole-cell immobilization in bioreactor

Inductive Surface Display

Surface display technology of induced Lactococcus lactis has been proved to be a very effective expression system and has been widely used in industry. We use genetic engineering to fuse the protein sequence provided by our customers into the transporter protein of Lactococcus lactis.

NOTE:

• We choose the surface anchoring domain of Lysine motif (LysM), namely the C terminal of ACMA (cAcmA), as the anchoring domain by default.
• Nisin system was used to induce the expression. In addition, we can also build phage, temperature, and pH induction system for customers. If you have specific requirements, please let us know before the formulation of the expression scheme.

Constitutive Surface Display

Compared with the inductive display, the constituent display system is more economical. We usually use ACMA as a heterologous protein anchor region and Usp45as secretory signal peptide.

Basic Principle

The basic principle of Creative BioMart'sLactococcus lactis surface display service is:

• Expression mission submission
• Selection of host strain and vector
• Construction of expression vector
• Plasmid transformation and positive clone screening
• Cell culturing and WB test

Didn't find the service you want? Please check out our Lactococcus lactis Bacterium-like particles (BLP) construction service. It is an inanimate particle based on non-recombinant Lactococcus lactis, which can be used as an immunostimulant to improve the existing vaccine and can be used for mucosal application, or as an alternative vaccine carrier for subunit antigen.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.