All known Yarrowia lipobytica strains are haploid and the genome of Y. lipolytica has been sequenced and annotated. Therefore, this yeast species has the potential for homologous and heterologous gene expression and secretion. To make usage of the excellent potential, Creative BioMart has developed a Yarrowia Lipolytica cell surface display service.
The basic mechanism of Yarrowia Lipolytica cell surface display is quite simple. The protein can be anchored on the cell surface by fusing with the cell well protein.
Among all the cell wall proteins of Y. lipolytica that have been isolated and characterized, protein Ylcwp1 is selected. The C-terminal part of Ylcwp1 contains the GPI anchor attachment signal sequence that allows the protein to be covalently bound to the β-1,6-glucans of the cell wall.
To conduct the display service, we select the Yarrowia lipolytica Po1f strain as the host yeast. Creative BioMart's technical team has developed a novel expression vector to mediate the display process. The vector is manipulated based on the pUC-hp4d vector. It contains
| Generation and amplification of expression vector | Timeline |
|
2-3 weeks |
| Linearization vector and electroporation into the host | |
|
1 week |
| Optimization of expression conditions | |
|
1 week |
| Amplification, expression and purification of target protein | |
|
1-2 weeks |
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