The recombinant protein expression system regulated by Trp promoter has been developed and matured gradually, and it can be used in different scales of high-level protein production. The operon is regulated so that when tryptophan is present in the environment, the tryptophan synthesis gene is not expressed. After induction, the transcription of the gene cloned from Trp promoter on plasmid increased about 50 times, and the gene product could reach 30% of the total cell protein. The promoter is easy to obtain and the activation conditions are simple, and can be used in the background of E. coli host. Creative BioMart's technical platform now provides Trp system-related services to clients in need.
We offer a variety of flexible service packages for the choice of the client.
Client only needs to provide the target sequence and requirements. We will be responsible for the steps including codon evaluation, expression vector design, induced expression, expression condition optimization, protein product detection, and large-scale production.
We can also build an expression vector regulated by Trp promoter for you. Our library contains hundreds of compatible plasmid carrier skeletons, which can build multiple vector candidates according to your needs.
If you encounter post-transcriptional problems in the experiment, you can consider our post-transcriptional problem guidance service. We will point out the possible problems for you and provide corresponding solutions.
The transcription of Trp promoter is controlled by intracellular free tryptophan level. Tryptophan binding to Trp apolipoprotein repressor can compete with RNA polymerase and bind to Trp promoter/operon. We usually choose wheel 3- β Indoleacetic acid (IAA) binds to Trp apolipoprotein repressor to form an inactive Trp pseudorepressor that does not bind to Trp operon. This allows the RNA polymerase to bind to the promoter and transcribe the gene of interest.
Below is the description of our basic services:
| Service Name | Service Description | Deliverable |
| Preparation of Trp vector backbone |
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| Preparation of target DNA |
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| Subclone construction |
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| Protein expression |
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| Scale up and protein purification |
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Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
