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Protein Production in Nicotiana Tabacum Expression System

Protein Production in Nicotiana Tabacum Expression System

In recent years, with the rapid development of DNA recombination technology and plant transgenic technology, transgenic plants have become a new type of bioreactor. At the same time, transgenic tobacco has made great progress as a research model expression system of transgenic plants. Tobacco has the longest history of producing recombinant protein, because by genetic engineering and has a high biological yield.

Protein Production in Nicotiana Tabacum Expression System

In addition, the tobacco expression platform is based on leaves, thus eliminating the need for flowering, greatly reducing the possibility of gene leakage into the environment through pollen or seed diffusion. Most importantly, tobacco is a kind of non-food and non-feed crop, which can reduce regulatory barriers to the greatest extent by eliminating the risk of plant recombinant protein entering the food chain. In different tobacco plants, in addition to producing a large amount of biomass and relatively few alkaloids, tobacco also produces the highest instantaneous concentration of recombinant protein, which makes it the most effective plant host for the production of recombinant protein.

Technology Description

Protein Production in Nicotiana Tabacum Expression System

Creative BioMart has developed a novel tobacco-specific expression platform for the production of high purity recombinant protein. Recombinant proteins expressed from plants usually have post-translational modifications, which are necessary to enhance protein stability, biological activity and good pharmacokinetics.

We can provide customers with a series of one-stop services, including vector construction, plant transformation, plant tissue culture, protein extraction and protein purification. Our services can fully meet the needs of customers and build a stable tobacco protein expression system. The specific construction methods of protein expression system are as follows:

Service step Description
Generation of genetic engineering plants
  • Gene optimization and synthesis, vector construction
  • Generation of transgenic plants
  • Screening for high expression lines
Lab-scale production
  • Screening the genetic stability of homozygous genetic engineering lines
  • Genetic analysis of genetic engineering line
  • Detection and activity analysis of recombinant protein
  • Developing a protocol for purification of the protein and providing a microgram-scale with 90-95% purity
Pilot-scale production
  • Providing milligrams or grams of protein with 90~95% purity
  • Providing data for biosafety assessment of transgenic plant

With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

References

  1. Garabagi F., McLean M.D., Hall J.C. (2012) Transient and Stable Expression of Antibodies in Nicotiana Species. In: Chames P. (eds) Antibody Engineering. Methods in Molecular Biology (Methods and Protocols), vol 907. Humana Press, Totowa, NJ.
  2. Molina-Hidalgo FJ, Vazquez-Vilar M, D'Andrea L, Demurtas OC, Fraser P, Giuliano G, Bock R, Orzáez D, Goossens A. Engineering Metabolism in Nicotiana Species: A Promising Future. Trends Biotechnol. 2020 Dec 17:S0167-7799(20)
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