WGE (Wheat Germ Extract) system is one of the most widely used eukaryotic CFPS (Cell-Free Protein Synthesis) systems. Compared with E. coli system, this technology has better performance in complex protein synthesis and post-translational modification. WGE is usually prepared from wheat seed embryos and reacts to produce hundreds of micrograms to milligrams of recombinant protein per milliliter, depending on the protein and form.
Click Here To See The comparison of each cell-free protein synthesis systems→
CFPE system of wheat germ extract is suitable for prokaryotic and eukaryotic protein expression. Codon optimization is usually not required even when expressing the high A / T or G / C content transcripts. The system is particularly suitable for the production of proteins with complex conformations and multiple protein complexes, such as disulfide bonds or intact membrane proteins. A variety of proteins and large proteins have been successfully produced from the WGE system.
| Part Of The Proteins That Successfully Expressed at Creative BioMart | |||||
| MeCP2 Protein | ADAMTS13 | Emerin | ERG | LDL Receptor | MDA5 Protein |
| 5HT7 Receptor | EBP50 | eNOS | Fbx32 | I Protein | MEIS1 Protein |
| 67kDa Laminin Receptor | EEA1 | Ephrin A1 | ID4 | 12 Lipoxygenase | MC4 Receptor Protein |
| ADA Protein | EED | ER81 | Jarid2 | MAFA Protein | MRC2 Protein |
Three WGE protein synthesis services are now available at Creative BioMart, each service has its features and application. Please click the links below for detailed information.
Based on the non-coupled WGE expression system, it is suitable for conventional protein synthesis. Linear or plasmid DNA was used as a template. Small / medium / large scale expressions are available as required.
It is designed for membrane protein expression. Large / small scale expression of membrane proteins can be achieved by plasmid template.
An efficient expression system based on SP6 promoter. Using the coupled expression system, the soluble full-length protein can be synthesized efficiently within 2 hours.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
