pET, a T7 promoter-regulated E. coli expression system has become the most popular system for protein production in the field of E. coli expression.
The T7 promoter is an 18 base pair DNA sequence that can be specifically recognized by T7 RNA polymerase. The pET system allows high-level expression and it is ideal for soluble, nontoxic recombinant proteins production in E. coli.
In order to improve the expression quality, Creative BioMart's technical team has reformed the commercial pET vector series. With the modified vector, we have successfully expressed a variety of foreign genes. The novel vector series have been successfully used in single strand induction, DNA sequencing and site-directed mutagenesis. The analysis of various expressed products showed that most of them are soluble and bioactive.
pET is a powerful prokaryotic protein expression system. After the target gene is cloned into pET plasmid vector, it is strongly transcribed and translated under the control of phage T7 signal under the induction of T7 RNA polymerase provided by host cells. The mechanism of T7 RNA polymerase is very effective and selective. After IPTG induction, almost all cell resources will be used for the expression of foreign proteins. Only a few hours after IPTG is added, the target protein is usually accounted for more than 50% of the total cell protein.
Here is the basic service principle. Please contact us for detailed information.
| Service Name | Service Description | Deliverable |
| Preparation of pET vector |
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| Preparation of target DNA |
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| Subclone construction |
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| Protein expression |
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| Scale-up and protein purification |
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E. coli can easily produce proteins that are difficult to get or purified from nature. In most cases, an ideal E. coli expression system should meet the following criteria:
(1) Low pre-induction leakage expression
(2) Fast and convenient induction methods
(3) It can express various genes efficiently
(4) Easy to be cloned
(5) Point mutation and DNA sequence analysis can be performed directly without subcloning
The test of expression products shows that our reformed pET expression system can meet the above requirements.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
