With the development of recombinant protein expression technology, the research and development of protein drugs have gradually become a hot spot. Peg is one of the commonly used macromolecular modifiers, which is widely used in the modification of protein/peptide drugs. It can solve the problems of short half-life and antigenicity of natural protein drugs.
Creative BioMart has developed its own protein modification technology platform, which can provide high-quality protein PEG modification services according to the needs of customers. We provide customers with high-quality one-stop service including information consultation, experiment scheme design and experiment implementation.
We recommend that you choose this PEG modification service as an additional service for our recombinant protein expression, so that we can provide you with more strict quality control and a more favorable price.
PEG is a synthetic polymer with high hydrophilicity. PEG can affect the spatial structure of protein and leads to the change of its biochemical properties. These changes include
The introduction of polyethylene glycol (PEG) into biomolecules and drugs can improve the stability and pharmacological properties of proteins, peptides, antibody-drug conjugates, and small molecule drugs.
PEGylation can increase the water solubility and stability of protein, and change its pharmacokinetics and immunogenicity. Creative BioMart can provide you with protein PEG modification, including:
PKD parameters, activity retention after protein modification, immunogenicity, modification efficiency, scalability and cost were used to evaluate and select the appropriate peg and modification scheme
Process scale-up and preparation of PEG-modified protein you need, develop an appropriate program to provide you with mg to G level of PEG-modified protein fermentation scale-up (GMP/GLP standard).
| Service step | Service description |
| PEG molecular selection | PEG raw materials are screened to obtain better pharmacokinetic characteristics |
| Biological activity screening | Select appropriate reaction groups base on the activity to ensure the specificity of the modified sites |
| Reaction system screening | Buffer system, pH, time, etc |
| Protein purification | The labeled protein is purified by affinity; The unlabeled protein is extracted by ion exchange column, density gradient centrifugation, molecular sieve and HPLC |
| Impurity removal | Establish a reasonable separation process and remove the residues of unmodified protein |
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
Reference
1. Radi, L., Fach, M., Montigny, M., Berger-Nicoletti, E., Tremel, W. and Wich, P., 2016. Methods of protein surface PEGylation under structure preservation for the emulsion-based formation of stable nanoparticles. MedChemComm, 7(9), pp.1738-1744.
