Subtilin-regulated Gene Expression Service

Bacillus subtilis is a widely studied Gram-positive bacterium. The system can express secreted protein without endotoxin. Up to now, nearly half of commercial protease and amylase are produced by Bacillus subtilis. There was no significant bias in the use of codons for Bacillus subtilis. In addition, Bacillus subtilis can secrete functional extracellular proteins directly into the culture medium (currently, about 60% of commercially available enzymes are produced by Bacillus subtilis). A lot of information about transcription, translation, protein folding and secretion mechanism, genetic manipulation and large-scale fermentation has been obtained.

Subtilin is a small peptide antibiotic composed of 32 amino acids produced by Bacillus subtilis. The subtilin-regulated gene expression service is now available at Creative BioMart.

Technology Description

The genes of the regulatory components called SpaR (response regulator) and SpaK (membrane sensor histidine kinase) can be isolated and placed on the chromosome of a B. subtilis host strain. One of the subtilin-regulated promoters is located upstream of a multiple cloning site into which the gene of interest can be cloned.

Upon addition of subtilin, the system is activated and the protein of interest is produced. It accumulates either intracellularly, or is secreted into the medium.

Basic Expression Principle

Service Advantages

• It has long been known that subtilin can achieve strict regulation of Bacillus subtilis
• Genome sequence available, rich in gene engineering experience, and a full range of genetic engineering tools
• The expression system is helpful to the expression of toxic protein products
• After insertion of the upstream chromosome of the relevant gene, it can be used to identify the necessary genes
• The expression system has the potential of secretory expression
• Gene controlled expression in metabolic engineering

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Creative BioMart's professional team will provide you with detailed guidance and solution ASAP.

Reference

1. Bongers RS, Veening JW, Van Wieringen M, Kuipers OP, Kleerebezem M. Development and characterization of a subtilin-regulated expression system in Bacillus subtilis: strict control of gene expression by addition of subtilin. Appl Environ Microbiol. 2005;71(12):8818-8824.