Yeast fermentation is the most economical eukaryotic expression system for the large-scale production of secretory and intracellular proteins that require post-translational modification, including humanized antibodies.
Features of yeast system:
Production of gram protein—— Yeast system is suitable for customized production of industrial enzymes.
High yield —— Compared with E. coli, protein production increased by 2 times. The yield of yeast fermentation is usually 5-10 m/L.
Stable production process—— The target protein expression cassette is integrated into the yeast genome to achieve a stable protein expression process
Click Here to See How to Select An Appropriate Protein Expression System→
Currently, two commonly used yeast expression systems are Pichia pastoris and Saccharomyces cerevisiae protein expression system. Both systems and their related services are available at Creative BioMart’s yeast technology platform.
Just tell us your requirement and submit the target sequence online, and we will complete the rest.
We provide P.pastoris and S. cerevisiae for your protein expression
Creative BioMart has decades of protein expression experience and 1000+ successful cases.
We use different yeast strains in different expression procedures to optimize your protein production.
We are equipped with professional technicians to discuss and optimize the experiment with you.
Yeast has the characteristics of fast growth and high protein production.
It is essential to use the appropriate host cell once you have decided to express your protein using yeast expression system. Although various yeast cell strains have shown their potential of being host cells, Pichia pastoris and Saccharomyces cerevisiae are still the most commonly used.
Among all the proteins secreted by Pichia pastoris, only a small proportion is the native protein. This property simplifies the downstream protein purification process.
Pichia pastoris can be cultured to reach high cell density, therefore, allowing for high productivity.
Pichia pastoris has the ability of processing complete eukaryotic cell PTM like phosphorylation and glycosylation.
Short culture time, high yield and mature technology for DNA manipulation.
S. cerevisiae has the ability to perform disulfide bond formation and most mammalian PTM.
Due to the GRAS status, S. cerevisiae is recognized as safe by the FDA
|
Expression vector construction
1 week
|
|
Small-Scale Protein Expression
4-5 weeks
|
|
Large-Scale Expression & Purification
2 weeks
|
|
Optional Services
|
Creative BioMart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.
