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Immunoassay Analysis Solutions

Immunoassay Analysis Solutions

Immunoassay Analysis Solutions

Immunoassay is a method for the quantitative or qualitative detection of proteins and other biochemical substances based on antigen-antibody specific binding. In order to meet the complex requirements from customers, Creative BioMart has developed a comprehensive non-clinical immunoassay solution for experimental research users. We can provide services including but not limited to:

Western Blotting (1-2 weeks)

Creative BioMart's technicians are skillful in sample processing, transfer and antibody dilution ratio, and can tailor the experimental scheme to customers' samples. The testing steps include:

  • Protein extraction and quantification
  • Discontinuous polyacrylamide gel electrophoresis
  • Protein transfer to nitrocellulose membrane or PVDF membrane
  • Membrane sealing and antibody incubation
  • Result detection and data analysis
  • Provide experimental report

We provide labeled secondary antibody and internal reference antibody α-Tublin, β-actin and GAPDH

ELISA (1-2 weeks)

It is a highly sensitive experimental technique based on immunological reaction, which combines the specific reaction of antigen and antibody with the efficient catalysis of enzyme to substrate.

In practical application, there are many methods and steps. We recommend double antibody sandwich method to detect unknown antigen and indirect method to detect unknown antibody

Immunoprecipitation (IP)

Based on the given gene sequences, expression plasmids, protein samples or tissue samples, we can provide:

Regular Co-IP service (1-2 weeks)

Mammalian protein expression +Co-IP+MS (verification of unknown protein) / silver staining (5-6 weeks)

MS / LC-MS + silver staining (1-2 weeks)

The materials we can deliver include gene synthesis report, original data, result picture, standard experiment report (including experiment steps and process), CO IP experiment report

Chromatin Immunoprecipitation (ChIP)

It is a powerful tool to study the interaction between protein and DNA in vivo. According to the cells or animal and plant tissues provided by customers, we can provide

ChIP qPCR detection

ChIP high throughput sequencing

The deliverable results include experimental report, qPCR results / high-throughput sequencing results, and original images.

Immunohistochemistry (IHC)

This technology is not only an important method for oncology research and diagnosis, but also has been widely used in various fields of medicine. Results from IHC have become an important part of the basis or diagnostic criteria for disease diagnosis. The services we can provide include ordinary (IHC include all these steps) tissue section, paraffin section, frozen section, tissue embedding, HE staining, MASSON staining, safranin solid green staining, Immunofluorescence staining.

Please note that

  • The detection is only for experimental research users, not for clinical diagnosis. The results are only for reference.
  • All immune testing services need customers to provide a sufficient amount of antigen and antibody, the second antibody will be provided by us.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

CONTACT OUR TEAM