Cell-Free Expression Platform

Cell-Free Protein Synthesis (CFPS)

Figure 1. Cell-free protein synthesis systems exploit crude cell extracts to produce valuable therapeutics and vaccines, among other products. (Cell-free protein synthesis: applications come of age, 2012)

CFPS is a synthetic biological technology. The technology uses the molecular translation mechanism in cell extracts to complete protein expression within a day. CFPS is an ideal method for functional protein translation in vitro. The cell-free gene expression reaction system consists of three parts:

• Cell Extract —— Contains ribosomes, RNA polymerase, and other transcription and translation accessory proteins derived from a source strain.
• Reaction Mixture —— Contains supplemented cofactors for protein synthesis (like amino acids, nucleotides, salts, polyamines, an energy source, molecular crowding agents, metabolic cofactors, buffer, transfer RNAs, and other metabolic additives)
• DNA Template —— Plasmid DNA or linear expression template obtained from PCR

The standard reaction volume is on the order of 10μL. The typical protein yield in the reaction is hundreds of mg/ml.

Creative BioMart's CFPS Platform

CFPS technology is suitable for a variety of downstream applications and is a trustworthy technology used in various research fields. At present, Creative BioMart has developed a cell-free expression platform, and we promise to provide satisfactory services for clients with mature technology and professional attitude.

Figure 2 . Comparison of in vivo recombinant DNA protein expression with CFPS (Cell-free gene expression: an expanded repertoire of applications, 2020)

From this platform, customers can find expression services based on cell extracts made from

In addition to the expression services, Creative BioMart also provides a series of cell-free protein expression kits based on the above cell extracts as products to customers around the world.

For detailed information, please go to our Service page.

Achievable Goals Based on the Platform

• Using the Cell-Free Protein Synthesis Technology, we can achieve the following goals for our clients:
• Expression conditions optimization. The lack of cell wall provides an open and universal environment for active monitoring, rapid sampling and direct manipulation of protein synthesis, which can artificially control the reaction conditions and process.
• Greatly shorten the expression period. The cell-based expression process takes at least 2-3 days in general, while cell-free expression requires only 1-2 hours.
• It can express proteins that are difficult to express, such as membrane proteins and toxin proteins.
• Preparation of protein complexes protein-protein interaction studies.
• Insertion of unnatural amino acids at specific sites, which is of great significance to the research and development of recombinant protein drugs.
• Virus-like particles (VLPs) expression for viral vaccine development

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.