Kluyveromyces lactis expression system has several advantages over other yeast and bacterial protein expression systems. The strain can rapidly reach high culture density and mass-produce recombinant proteins. It has been used in industrial-scale protein production in the food industry for more than 10 years ago.
For clients who intended to use K. lactic strain for large-scale industrial fermentation, we recommend Creative BioMart's Integrated Kluyveromyces Lactis Expression System Construction Service. In order to establish a cell line stably expressing the foreign protein, we can integrate the foreign gene into the chromosome of the host yeast by using an integrated vector that harbors homologous regions of the K. lactis chromosome.
We use a simple homologous recombination method to construct cell lines and express proteins (intracellular or secretory). The traditional K. lactis expression vector is optimized and applied to our expression protocol. We use a strong LAC4 promoter to initiate gene expression. The expression vector contains an α-mating factor secret domain (α-MF) for secretory expression. The genes inserted downstream of α-MF are processed and secreted in the Golgi apparatus.
The construction and copy of subclones need the participation of E.coli. After the linearized recombinant vector is transformed into Kluyveromyces lactis competent cells, the foreign fragment could be integrated into the LAC4 locus of the Kluyveromyces lactis genome.
Our service is very flexible. Our main purpose is to make customers satisfied. Clients can start our expression project by submitting the target sequence online. By default, we use our original vector for cell line construction. Of course, you can also provide your vector and template sequence by mail or authorize us to purchase from other companies.
As the program is completed, clients will get (1) a vector sequencing report; (2) a protein expression test report; (3) a package that contains the recombinant expression vector, recombinant cell strain, and target protein of required concentration.
The integrated expression system construction service is suitable for clients who want to use K. lactic strain for large-scale industrial fermentation. If it does not fit with your requirement, we also offer Episomal Kluyveromyces Lactis Expression Service, which is less stable but more suitable for rapid protein expression.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
