Transfection is the most essential step of stable cell line development. Transfection can be mediated by virus vectors, plasmid vectors or some non-vector methods. Creative Biomart's mammalian expression platform is competent for most commonly used non-vector mediated transfection. Each of them has its unique features and application.
Positively charged liposomes combine with DNA by electrostatic interaction to form DNA liposome complexes, which then enter cells through endocytosis.
The positively charged cationic polymer and the phosphate group of nucleic acid form a positively charged complex. The complex enters the cell by endocytosis.
It is similar to liposome transformation, but it has lower toxicity, simple operation and wide applicability.
Calcium phosphate can promote the combination of exogenous DNA and cells, and calcium phosphate DNA complex can adhere to the cell surface and enter the cell through endocytosis.
The high pulse voltage destroys the cell membrane and forms pores on the surface, and DNA enters the cell from the pore.
DNA is injected directly into cells by microsystems and microinjection techniques
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