Stable Isotope Labeling Service

Protein isotope labeling is a classical technique for protein tracing and proteomics quantitative analysis. The essential amino acids labeled with natural isotope (light) or stable isotope (heavy) are used to replace the corresponding amino acids in cell culture medium, so that the newly synthesized proteins can be labeled by adding amino acids containing different isotopes during cell growth.

In order to achieve strict quality and process control, Creative BioMart has established its own isotope labeling platform. 15N, 13C or a combination of 15N and 13C are used by default.

Isotope labeling can be applied to proteins produced by various expression systems including bacteria, baculovirus, yeast and mammalian systems. Clients can also authorize us to perform one-stop protein expression and labeling services.

Basic Labeling Principle

• The project determines the basic expression system
• According to the characteristics of the system, the target protein sequence analysis and vector selection are carried out
• Construction, cloning and small-scale expression test of vector
• Host cell culture and cell labeling
• The target protein is expressed in cells
• Sample preparation, including protein extraction, mixing unlabeled (light) and labeled (heavy) samples, trypsin digestion, peptide fractionation.
• Using professional software and database for data analysis, the results are reliable.
• Detailed report.
• Product delivery

In addition to 15N and 13C, other isotopes like 125I, 131I, 2H, 3H and 35S are also available if required.

Protein isotope labeling service can also use 125I and 131I. Tyrosine in protein can be labeled by HPLC or open tubular chromatography (depending on the characteristics of iodized molecules), and free iodine in protein can be removed. Radioiodine can be labeled on protein and other molecules, and radioiodine can be used as a tracer or probe. In general, the TLC method is used to detect the labeled products to ensure that the percentage of free iodine is less than 5%.

Things You Should Know Before the Project Start

Protein labeling reaction is a reversible chemical reaction, and there is an essential consumption. When there are too few reactants, the success rate of the experiment will be affected; at the same time, the labeling efficiency can not reach 100%.

Sample requirements: the labeled antibody/protein shall be at least 1mg, the purity shall be more than 90%, and the concentration shall not be less than 1mg/ml.

The protein labeling service does not include the purity determination of the labeled products and the detection of the working concentration of the labeled products. If you need further detection of the modified products, please communicate with the technical support personnel.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.