Saccharomyces cerevisiae has become an important tool for protein expression research, industry and medical applications. As a food organism, it is useful for protein production. The yeast can grow rapidly on a simple medium, with high cell density, and its genetics is more advanced than other eukaryotes, so yeast can be manipulated almost as easily as E.coli. As eukaryotic, Saccharomyces cerevisiae is a host organism suitable for high-level production of secreted and soluble cytoplasmic proteins.
Creative BioMart's yeast expression platform can provide comprehensive expression services.
In our technology platform, using Saccharomyces cerevisiae to express protein involves the following steps:
Based on the basic expression principle above, we offer four types of expression vectors for customers to choose from. Each vector has its features and applications.
Yeast Integrating plasmids (YIp)——The plasmid lacks ori, thus must be directly integrated into the host chromosome by homologous recombination.
Yeast Centromere plasmids (YCp)——It is considered as a low copy vector and combines partial ARS and partial centromeric sequence (CEN)
Yeast Episomal plasmids (YEp)—— It is considered to be a high copy vector which is similar to bacterial plasmids. A fragment from the 2-micron circle allows for 50+ copies to stably propagate per cell.
On top of the classic expression service mediated by the YIp, YCp and YEp vector, the services we can offer include but are not limited to:
If you have any other expression requirements, please let us know. We will try our best to provide you with satisfactory service.
Did not find what you need? In addition to the Saccharomyces Cerevisiae Protein Expression Service, we also offer
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
