Based on phage display technology, Creative BioMart can provide our clients with natural, semi-synthetic, and fully synthetic peptide libraries as well as various types of cDNA libraries.
The development of next-generation sequencing (NGS) technology makes peptide library a very powerful tool. Nowadays, peptide libraries are usually used to explore protein-protein interaction, antigen-antibody interaction, substrate enzyme interaction, intracellular protein network mapping, high-throughput and high-content screening.
At Creative BioMart, we use phage display technology to build a highly diverse, natural, semi synthetic and fully synthetic peptide library for scientists. Here, we use the modified M13 phage as the delivery system. The modified vector can be modified and contains the starting point of bacteriophage and bacteria replication, the signal of bacteriophage packaging, the selective marker gene and the selected fusion protein gene.
In the development of peptide ligands and epitope mapping, we will carry out affinity selection and amplification of phages for 2 to 4 iterations, and then use ELISA to screen dozens of positive clones and sequence them. After screening, candidate peptides will be synthesized and their binding ability and biological activity of protein targets will be tested.
In the past few decades, the cDNA library established by phage display technology has been widely used in antigen/antibody discovery, vaccine design, clinical treatment and diagnosis. The whole cDNA product can be expressed on the phage surface by fusion protein, thus identifying a large number of candidate proteins and separating specific binding agents for various targets.
Creative BioMart has the ability to build a variety of high-quality cDNA libraries with high capacity, high density and correct direction by improving its technology platform.
We can build a library with at least 3.1 × 106 primary clones. The average insertion size is at least 1 KB. All clones have been correctly targeted and can be used for expression, antibody screening or isolation of specific cDNA clones.
This is a powerful tool for rare gene discovery. It can be used to improve the universality of low abundance clone, and eliminate the bias of sequencing effectively.
If you need to get a high proportion of low abundance full-length genes, consider a full-length cDNA library.
The technique is suitable for low-level genes. The abundance of unrelated sequences can be reduced by 10% to 90%.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
