Yeast one-hybrid technology is a tool for studying nucleic acid-protein interaction based on yeast two-hybrid technology. It is widely used to study gene expression regulation in eukaryotic cells, such as identifying DNA binding sites, finding potential binding protein genes, analyzing DNA binding domain information, etc.
Creative BioMart has rich experience in yeast library construction and library screening, which can help customers analyze and solve various problems encountered during experiments.
Figure 1. Yeast one-hybrid (Y1H) assays (Sewell, 2018)
The experimental process of yeast one-hybrid is simple and time-saving. It can directly identify and find out the protein and its coding sequence combined with the specific acting element, without the tedious biochemical means such as preparation and purification of protein or antibody to establish this interactive relationship.
In addition, yeast belongs to prokaryote, so the research in yeast is closer to the real situation of eukaryotic gene expression regulation, and the protein is in natural conformation, which avoids the lack of in vitro research.
Figure 2. Outline of variations and considerations in Y1H assays (Sewell, 2018)
We provide library construction and library screening services:
| Service project | Customers provide | Deliverables |
| Library construction | Tissues/Cells | Liquid of yeast library (titrated higher than 1 x 108 Pictures of PCR identification results of library (with minimum 80% positive rate) |
| Library screening | Promoter gene sequence / plasmid | All original pictures in the screening process; Screening and sequencing results of all interacting prey proteins |
Library construction
Library screening
The results of the yeast one-hybrid experiment can be further verified:
| Service project | Objective | Details | Timeline (weeks) |
| EMSA | Verify the interaction between promoter and protein | Probe synthesis and labeling | 10 |
| EMSA test | 20 | ||
| Double luciferase detection | Verify the interaction between promoter and protein | Vector construction | 10-15 |
| Double luciferase detection | 25 |
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
Reference
1. Sewell JA, Fuxman Bass JI. Options and Considerations When Using a Yeast One-Hybrid System. Methods Mol Biol. 2018;1794:119-130.
