Cell-free protein synthesis (CFPS) has become a powerful tool for expressing proteins in vivo. The E.coli is one of the most commonly used cell species for extract preparation and it is the first system developed for cell-free protein synthesis. After several improvements, E.coli cell-free expression system has become a powerful, cost-effective, multifunctional and scalable technology, which can be used to produce proteins, such as vaccines, antibodies, cytokines, and paves the way for the emerging field of cell-free metabolic engineering (CFME).
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The basic principle of E. coli cell-free protein synthesis system is:
Two cell-free E. coli protein synthesis services are now available at Creative BioMart, each service has its features and application. Please click the links below for detailed information.
Use prokaryotic E. coli-like promoters such as lacUV5, tac, λPL (con) and λ-PR. It is suitable for clients who provide linear or circular DNA as template.
This is a high-yield cell-free system. Use T7 RNA polymerase to enhance productivity. The template can be provided in the form of plasmid DNA, Liner DNA or mRNA.
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