Chlamydomonas has the characteristics of simple structure, easy culture, clear genetic background, edible and endotoxin-free, which is a potential foreign protein expression system. At present, Chlamydomonas reinhardtii has become the most mature expression system in eukaryotic microalgae.
Creative BioMart has established a mature Chloroplast Expression System of Chlamydomonas reinhardtii after many years of effort. We have taken over hundreds of expression cases and successfully expressed a variety of reporter genes, medicinal proteins, and enzymes for our clients.
The expression protocol we developed was based on the chloroplast of Chlamydomonas. The whole method of genetic transformation is similar to plant genetic transformation. In order to obtain transgenic cell lines, foreign genes are introduced into the chloroplasts of algae by particle bombardment.
Specifically, our service process mainly includes:
| Service Name | Service Description |
| Expression strategy design | Client provides the target sequence and expression strategy designed referring to customers' requirements and protein properties |
| Codon optimization and gene synthesis (1 week) | Optimize the codon in order to improve protein yield significantly Use PCR to amplify the target fragment and link it to the expression vector |
| Construction of the expression cell strain (2 weeks) |
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| Protein expression, evaluation and optimization (1 week) |
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| Scale-up and protein purification (2-3 weeks) | We will scale up your protein expression using optimized conditions. After multistep protein purification, the protein purity will be >90% |
| Deliverable |
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Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
