Protein Production in Suspension Cell System

Plant suspension culture has become a promising alternative biological production platform for recombinant proteins, which combines the advantages of whole plant production, microbial fermentation and mammalian cell culture. Compared with the traditional expression system, it has many advantages, such as its inherent security, cost-effective biological processing and post-translational modification ability. Many kmedicinal protein have been successfully produced in plants, including antibodies, vaccines, hormones, growth factors, cytokines and therapeutic enzymes.

What We Can Do For You?

Over the years, Creative BioMart has been committed to optimizing protein expression platforms based on plant suspension cells, producing recombinant proteins, peptides and industrial proteins for customers around the world. With many years of experience in plant tissue culture and protein expression, Creative BioMart provides a comprehensive service from codon optimization, molecular construction, plant cell transformation to protein expression and purification. Our mission is to help you create complex proteins, antibodies and vaccines using unique plant expression platforms.

For clients who want to express proteins in suspension cells, we recommend Tobacco BY-2 (Bright yellow-2) and NT-1 (Nicotiana tabacum-1) cell lines due to their robust and fast growth. If required, we can also provide other commonly used cell lines include rice, alfalfa, tomato, soybean, and carrot.

*PPlease note that this service is only suitable for clients who need the recombination protein. The final product delivered is only the target protein. If you are looking for the Suspension Cell Expression System Construction Service, please go to the related page.

Basic Workflow

• Preparation of the aseptic seeding
• Callus induction and transformation
• Cell suspension culture in bioreactor
• Extraction of plant-made protein
• Protein purification and formulation

Why Express Your Recombinant Protein in Suspension Culture System

Compared with other plant-based production platforms that have been explored, the advantages of plant cell suspension culture are as follows:

• It completely avoids the problems related to the environment, weather, pests, soil and gene flow.
• The growth cycle is short. The time scale needed to produce recombinant protein in plant cell culture can be calculated within a few days or weeks after transformation, while it takes several months in transgenic plants.
• The fusion protein can be expressed and secreted into the culture medium. It can be recovered and purified without a large amount of contaminated protein
• The downstream processing of protein extracted from plant cell culture medium is cheaper, which can balance the higher fermentation cost.
• High security. When needed clinically, recombinant proteins can be easily produced in ssuspension-cultured plant cells under controlled and sterile conditions

With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcomed to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.