Conjugated Enzyme Labeling Service

Creative BioMart can label the target protein by enzyme/antibody coupling. Our service aim is to provide high-quality protein labeling services for the research community through customized labeling services.

Conjugated Enzyme Labeling Service Figure 1. Enzymatic protein labeling techniques (Zhang, 2018)

We have constructed an enzyme coupling platform compatible with various enzymes and proteins/antibodies. The platform has high turnover, stability, easy coupling and relatively low cost. Through continuous improvement, the ratio of enzyme to protein/antibody analogs has reached about 1:1, which can maximize the retention of enzyme and carrier protein/antibody activity.

Available enzyme conjugates

Here are a few of our commonly used enzymes and their advantages and disadvantages.

Horseradish peroxidase (HRP)

HRP is composed of several isoenzymes with a molecular weight of 40000 and an isoelectric point of pH 3-9. The suitable pH for enzyme catalysis is slightly different due to different hydrogen donors, but most of them are around pH 5.

HRP is suitable for rapid color reaction, and the OD450 value can reach about 1.8-2.5 in 5-10 minutes when adding substrate in ELISA.

Horseradish peroxidase

Alkaline phosphatase (AP)

AP marker is suitable for slow color reaction. The color time is about 4-8 hours, and the Yellow compounds formed by catalysis are stable, and suitable for quantitative detection of enzyme-promoted kinetics.

Alkaline phosphatase

β-galactosidase

Enzyme	Strengths	Weakness	Detection Method	Application
HRP	Substrate suitable for immunohistochemistry, 1:1 coupling	The level of endogenous enzymes in blood cells was high and the sensitivity of soluble substrate is low	Soluble-TMB	Immunoassay
			Insoluble- chloronaphthol DAB/cobalt	Immunohistochemistry, Western blot
AP	The soluble substrate is good, suitable for Western blotting, and had hypersensitive substrate	Some tissues have endogenous enzymes, and the molecules connected are large	Soluble-PNPP	Immunoassay
			Insoluble-BCIP/NBT	Western blot
β-galactosidase	The endogenous enzyme activity is low and the substrate is good	It is easy to inactivate and the linked molecules are large	Soluble-ONPG	Immunoassay
			Insoluble-BCIG	Western blotting, immunocytochemistry of blood samples

Technology Applications

ELISA
Blotting techniques
In situ hybridizations
Immunocytochemistry
Immunohistochemistry.

Service Advantages

Strict quality control system, skilled laboratory technicians, rich marking experience;
There are many kinds of markers and labeling methods to choose from;
Mature technology routes and optimized experimental processes ensure a high success rate of protein labeling;
Improve the detection sensitivity and simplify the detection process;
Complete upstream and downstream services: it can provide one-stop technical services such as recombinant protein expression and preparation, protein sequencing, protein labeling modification, etc., saving customers precious time and cost;
Service can be customized: according to customer needs, provide customers with information consulting, detailed design and implementation of the experimental program and other related services

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

Reference

1. Zhang, Y., Park, K., Suazo, K. and Distefano, M., 2018. Recent progress in enzymatic protein labelling techniques and their applications. Chemical Society Reviews, 47(24), pp.9106-9136.

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