Bacillus subtilis Auto-Inducible expression Service

Creative BioMart's technical team has developed a service to automatically induce Bacillus subtilis expression. This technology has greatly optimized the sugar-induced Bacillus subtilis expression system, which enables us to obtain higher product yield and excellent biological activity at the lowest cost.

Technology Description

At present, the system has successfully expressed dozens of antibodies, vaccines and functional peptides for clients. With our reliable platform, researchers will get the most valuable biological products in the world. The Auto-inducible Bacillus subtilis Expression Service at Creative BioMart is dedicated to improving the yield greatly with a sophisticated combination of the novel auto-inducible system and high-cell-density fermentation. In addition to the diligent optimizations and professional experiences, our labs guarantee a series of most professional services.

The standard Bacillus subtilis system before modification has been induced by the expensive mannose operon positive-regulated manP promoter. Inspired by some natural automatic induction Bacillus subtilis systems (PST operon system, gsiB system and lysine ribose switch system), our technical team has developed new automatic induction Bacillus subtilis expression services. The automatic induction system manipulates the mannose operon through the deletion of manP gene, leading to the structural activation of mannose operon in the absence of mannose. For example, TQ356 represents a common strain of automatic induction systems with improved yield. High-density culture technology further improved its expression and accumulation efficiency.

Service Advantages

• Adopt an auto-inducible strategy to bypass the inducer problem.
• Combine high-cell-density culture technique to elevate yield of 5-folds.
• Multiple excellent strains and vector systems for regular and toxic products via intracellular accumulation or secretion expression.
• Optimal cultivation and induction are available with the whole range of scales from 5 mL to 1000 L per batch for the conventional amylases, proteases, lipases, riboflavin, etc., and multiple antibodies (conventional antibodies and novel intrabodies), vaccines, functional peptides, and other biopharmaceuticals, with reliable and superior yields and bioactivities.
• One-stop service: from initial design and optimization to purification and further modification with well-recorded data and organized (start a new point): High success rate: Successfully generated nearly a hundred proteins and antibodies in the past decade.

Related Service

Not the service you want? We also offer sugar induced Bacillus subtilis expression system. Please click the link if you are interested.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Creative BioMart's professional team will provide you with detailed guidance and solution ASAP.