Creative BioMart's cell-free expression platform can provide clients with perfect technical services. Cell-free protein expression system can carry out protein synthesis by simulating the life phenomenon of biological cells (like intracellular protein transcription and translation). Cell-free protein synthesis system uses exogenous target mRNA or DNA as the template for protein synthesis.
Figure 1. Schematic representation of a CFPS system (Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems, 2019)
By artificially controlling the addition of substrates, transcription and translation-related protein factors, the target protein can be synthesized in vitro.
*Click Here to See How to Select An Appropriate Protein Expression System→
Currently, we are able to provide various cell-free protein synthesis services using different cell extracts. These services are:
| Cell Extracts | Related Services |
| E. coli | Cell-free Protein Synthesis in E. coli Lysate |
| Rabbit Reticulocyte | Cell-free Protein Synthesis in Rabbit Reticulocyte Lysate |
| Wheat Germ | Cell-free Protein Synthesis in Wheat Germ Lysate |
| Insect Cell | Cell-free Protein Synthesis in T7 insect cell Lysate |
| Mammalian Cell | Cell-free Protein Synthesis in Mammalian Cell Lysate |
| Yeast Cell | Cell-free Protein Synthesis in Yeast Cell Lysate |
| PURE | Cell-free Protein Synthesis in PURE System |
| Other | Membrane Protein Expression Based on CFPS |
*Need assistance on your decision making? Click Here To See The comparison of each cell-free protein synthesis system→
Creative BioMart's professional technical team can solve the problems related to protein expression according to the special properties of different target proteins, for example, low protein production, expression of special proteins (such as membrane proteins, toxic proteins, etc.), preparation of protein complexes, parallel synthesis of a variety of different proteins, etc.
1. Target protein information or gene sequence, plasmids (we can provide plasmids or you can authorize us to purchase plasmids for you.);
2. Protein inquiry information sheet
1. Protein with a purity of more than 90%;
2. Protein expression report, including sequencing report, experimental conditions, protein expression purification test results.
The following is the basic service process and time consumption.
| Service Step | Timeline |
| Gene optimization and synthesis | 2-3 days |
| Subcloning | < 1week |
| Small scale expression condition test | 1 week |
| Protein preparation | < 1week |
| Protein purification | 1 week |
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
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