Protein Production In Rice Endosperm Specific System

Rice is the staple food of more than half of the world population, and has no potential allergen. The possibility of protein expression in the endosperm is very low to be degraded by protease. Meanwhile, the technology of the rice endosperm expression system has become mature, which is a suitable tool for expressing recombinant proteins. At present, the system has been widely used and eexpressed in various recombinant proteins.

Creative BioMart has successfully produced various recombinant proteins for academic and biotechnology industries by using rice endosperm cells as bioreactors. The applications of these proteins include but are not limited to: pharmaceutical products, antibodies, therapeutic proteins, industrial biological enzymes, human nutrition and health products, safe cell culture medium supplements, cosmetics and skincare products.

Service Description

Service Steps	Description
Generation of ggenetically modified crops	Codon optimization, gene synthesis, selection of endosperm-specific promoter, and vector construction Agrobacterium-mediated rice transformation Molecular characterization Screening the transgenic line of high-level expression
Lab-scale	Screening the homozygous lines that ppossess a stable inheritance Bioactivity analysis of recombinant protein Recombination proteins of microgram scale with 90-95% purity
Pilot-scale	Milligrams or grams of recombination proteins with 95% purity Biosafety analysis of GMOs (genetically modified crops)
Large-scale	Large-scale recombinant protein production

Protein Production In Rice Endosperm Specific System

Why Choose Rice Endosperm Specific System

High yield: compared with other plant expression systems, the yield is 10 times higher.
SSafety: oral products produced in oral safety factories.
Eukaryotic expression system: rice can produce complex biological agents, including proteins requiring post-translational modification.
Flexibility: the ability to produce multi-subunit molecules, monoclonal antibodies, fusion proteins and enzymes.
Purity: natural products without animal, human or microbial by-products, making it an ideal choice for therapeutic use or sensitive and strict biotechnology applications.
CCost-effectiveness: it is suitable for cGMP production, and the production cost is greatly reduced.
Scalability: the production of recombinant protein ranges from kg to hundreds of tons.
Storage: mature rice can be stored without product degradation.
Biological safety: rice is self-pollinated to maintain gene purity, and there is a monitoring chain from the target gene to the final biological gene.

With our professional research and development team and the latest protein expression and purification resources, we promise tto deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

Reference

Xu, R., Li, D., Li, H. et al. Isolation of four rice seed-specific promoters and evaluation of endosperm activity. Plant Cell Tiss Organ Cult 128, 125–132 (2017).

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