Baculovirus-Mediated Mammalian Surface Display Service

The fact that baculovirus can enter other cell types without causing any cytopathic damage makes baculovirus a perfect tool for heterologous modification. Through being inserted downstream of appropriate promoters (which can be recognized by mammalian cells or other cells), the target sequence can be expressed in cells of other species mediated by baculovirus.

Based on the BacMam system, the gene of interest can also be displayed on the surface of mammalian and other non-lepidopteran cells. This service is suitable for customers who want to express pharmacological cell-based assays for cellular receptors, transporters, ion channels, and viral targets. 

Service Description

Baculoviruses can carry out the protein display in cells of the following organisms: human, rabbit, non-human primate, rodent, porcine, bovine, ovine mammalian cells, fish, and mosquito.

Baculovirus-mediated surface display greatly relies on the interaction of the virus GP64 with mammalian surface phospholipids. The target gene is inserted into the full-length or part of GP64 expression cassette, meanwhile, there is an upstream CMV or SV40 promoter which could be recognized by mammalian cells. In this way, the foreign gene expressed in mammalian cells will be guided and anchored in the mammalian cell membrane.

Creative BioMart provides three forms of fusion options for customers to choose from.

• Solution 1: Clone the foreign protein-coding sequence into the expression cassette with its full-length coding region which includes signal peptide and transmembrane domain
• Solution 2: Clone the sequence into a truncated GP64-fused form
• Solution 3: Clone the sequence into a truncated form fused to foreign anchor domains
• Solution 4: Clone the sequence into a truncated GP64 which contains VSVG

The foreign fragment is inserted into polyhedrin through the routine procedure and displayed on the occlusion body. The location of the protein display is shown in the figure above

Service Features

Compared with other expression systems, BacMam has the following advantages:

• Safe – Low biological hazard, does not replicate in mammalian cells and has no cytopathic effect
• Low toxicity – Ability to reduce overt cytotoxicity
• Efficient – Has high transduction efficiency for mammalian cell lines, primary cell lines and stem cells
• Wide host range – Can be transferred into many mammalian cell lines
• Regulatory – Gene expression can be easily regulated by changing the amount of transduction

Recommended Applications

• Rapid – Expression is much faster than the generation of stable cell lines
• Delivery vectors for genes in mammalian cells. Including nuclear receptors, secreted proteins and membrane proteins, such as G protein-coupled receptors (GPCR), drug transporters and ion channels.
• Functional characterization and protein production for structural studies
• Cell membranes production
• High content screening assay development
• Vaccine production