Hansenula Polymorpha is methylotrophic yeast. It has been proved to be an effective host for the production of foreign proteins. It has a variety of unique functions, including the use of strong promoters of methanol metabolism-related genes, stable maintenance of multiple copies of foreign genes in chromosomes, and easy growth to 100-130 g / L of high cell density. H. polymorpha is also a useful biocatalyst for metabolic pathway engineering.
Creative BioMart has developed a cell surface display system based on Hansenula polymorpha. Our technology platform is able to provide the surface display service mediated by HpSED1, HpGAS1, HpTIP1, and HpCWP1 cell wall c.
The basic mechanism of Hansenula Polymorpha cell surface display is simple. The protein can be anchored on the cell surface by fusing with the cell well protein.
To develop our display protocol, we adopted a novel expression vector that contains a MOX promoter, a GAPDH promoter, α‐amylase signal sequence, killer toxin signal sequence and an unknown terminator. The LEU2 deficient DL1strain of H. polymorpha is selected as the host.
| Generation and amplification of expression vector | Timeline |
|
2-3 weeks |
| Linearization vector and electroporation into the host | |
|
1 week |
| Optimization of expression conditions | |
|
1 week |
| Amplification, expression and purification of the target protein | |
|
1-2 weeks |
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