Pichia Pastoris Protein Expression Service

Yeast expression system has the advantages of both prokaryotic and higher eukaryotic systems. It has the advantages of

It is widely used in the production of recombinant protein, especially eukaryotic protein. Pichia pastoris is the first choice as it can express recombinant protein because of its fast growth, abundant commercial expression vectors and high-efficiency secretory expression.

Creative BioMart has established a mature yeast expression and purification service platform to provide high-quality recombinant protein expression and purification services in Pichia pastoris.

Creative BioMart’s Improved Pichia Pastoris Expression Service

Creative BioMart's Yeast Expression system delivers yields of up to 12 g/L of post-translationally modified recombinant protein. This system can achieve:

• Protein yields up to 12 g/L
• Scalable production
• Convenient kit formats
• Post-translational modifications via eukaryotic expression

This system contains both low and high copies of plasmid backbones, 8 secretion signal sequences and 4 yeast strains—all to help you optimize for highest yield possible of your recombinant protein.

Service Features

• Plasmid copy number: both high- and low-copy options enable optimization of toxic protein expression
• Number of secretion signal leader sequences: 8 sequences
• Number of strains: 4 strains
• Number of protease-deficient host strains: 3 strains
• Selection method: adenine selection
• Obtain functional proteins: P. pastoris has many eukaryotic post-translation modification processes, offering a high probability of an active function recombinant protein.
• The advantages of P. Pastoris include rapid growth, a well-defined genetic background, simple media formulations and handling techniques--all of which make it an ideal host for producing large quantities of protein in a short period of time.
• Scalable expression to meet your needs: Using the P. pastoris system for small scale-up to fermentation-scale production helps ensure you have the flexibility to produce the protein quantity you need for your application.

What We Can Do For You

1. Construct recombinant yeast expression plasmid

2. Electroporate recombinant plasmid

3. Screen recombinants

4. Small-scale protein expression and purification (2L medium culture product);

5. Large-scale protein expression and purification (more than 10L).

STEP	SERVICE NAME	TIMELINE	DESCRIPTION
1	Construction of recombinant yeast expression plasmid	2 Weeks	Different vectors, different affinity tags, secretory or intracellular expression can be selected
2	Electroporation of recombinant plasmid	1 Week	5-10 clones can be identified by PCR
3	Screening of recombinant expression vectors	3-4 Weeks	Screen 5-10 clones. The recombinant is screened by zeocin concentration, and the expression of recombinant protein will be identified by Western blot
4	Small scale protein expression and purification	2-4 Weeks	The medium, cell density, methanol concentration and induction time are optimized
5	Large scale protein expression and purification	According to the expression volume	Shake flask or fermentor

Related Services

• Membrane Protein Expression in Pichia Pastoris
• Classic Pichia Pastoris Protein Expression Service
• Glycoprotein Expression in Pichia Pastoris

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.