When you obtain a monoclonal antibody produced by hybridoma cells (or other pathways) but do not know its DNA sequence, please consider Creative BioMart's monoclonal antibody sequencing service. Based on Rapid Amplification of cDNA Ends Technology (RACE), our comprehensive monoclonal antibody sequencing service includes:
If requested, our technology team is able to clone and sequence monoclonal antibodies from a wide range of species. Just contact and tell us your requirement.
If you plan to apply for a protective monoclonal antibody patent, you need to provide the antibody sequence to prove that your antibody is unique.
In order to eliminate the limitations of hybridoma technology, the obtained antibody sequence can be subcloned into mammalian cells to achieve stable expression. See our recombinant antibody expression service→.
If you want to express multiple sequences at the same time, Creative BioMart's high-throughput antibody expression service will be able to help you.
Over 2000 antibodies have been successfully cloned and sequenced with our optimized PCR primers.
Optimized workflow is used to clone and sequence the antibody. Antibody PCR, clone, sequencing and analysis can be done within 2 weeks.
Multiple clients will be processed at the same time.
The general procedure of 5' RACE-based cloning and sequencing antibody variable regions of hybridoma or clonal B cells:
| Customers provide | Service procedure | Timeline | Deliverable |
| 100000 cells per hybridoma clone are sufficient for cloning and sequencing antibody genes. | Extract and purify total RNAs from hybridoma or clonal B cells | 1-2 days |
Inquiry |
| Synthesize cDNAs | 1-2 days | ||
| Amplify DNA fragments including VH and VL, leader sequences, and partial constant regions (CH and CL) using 5' RACE; | 3-5 days | ||
| Subclone PCR positive bands | 1 week | ||
| Sequence 10 clones in total | |||
| Perform CDR analysis using sequencing data. |
Creative BioMart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.
