In the study of genetic engineering, it is often required that the expression of foreign genes should be strictly sequential, especially when the product is either cytotoxic or cytostatic. In order to realize the regulation of the spatiotemporal and expression level of exogenous gene expression, a variety of inducible mammalian gene expression systems came into being. These systems have been applied to a wide range of research areas including functional genomics, gene therapy, tissue engineering, biopharmaceutical protein production and drug discovery.
Creative Biomart provides several inducible systems that are mainly based on bacterial gene control elements and viral transactivator proteins. Each system has its own features.
Here are some inducible mammalian protein expression systems as well as some expression regulation methods currently available at Creative Biomart. Please click the links below to browse for more detailed information if you are interested. We are always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.
Cre-Lox Recombination allows DNA modifications to be targeted to specific cell types or triggered by specific external stimuli.
Bacterial elements Tn10 tetracycline-resistance operon is involved in regulated protein expression in mammalian cells. Very high induction rations had been reported.
A widely used protein expression regulation method. Heavy metal ions are applied to regulate the reaction.
IFNs are pleiotropic cytokines. They are inducible in many kinds of mammalian cells.
Based on synthetic ecdysone induced receptors and synthetic receptor recognition elements that regulate gene expression. The function of retrovirus is further expanded by completely controlling the retrovirus version of the system, and the induction rate in tissue culture cells is more than 1000 times.
Can positively or negatively regulate the variety of gene expression by binding to intracellular glucocorticoid receptors (GRs).
A relatively novel system which is established based on murine β-globin promoter globin LCR and murine erythroleukemia (MEL) cells. The step of clone selection and gene amplification are eliminated. Especially suitable for laboratory-scale recombination protein production. Can also be used to produce cytotoxic or cytostatic proteins.
