Transient mammalian expression system is one of the common mammalian protein expression patterns. It can produce a certain amount of recombinant protein/antibody in a short time. Transient expression system is efficient and flexible, so it is widely used in the rapid expression of gene products and small-scale preparation of proteins.
Creative BioMart's transient mammalian expression service usually starts with the analysis of the target sequence provided by customers and matches the most appropriate expression vector and expression pathway to ensure at least a 95% success rate. At present, we mainly use CHO and HEK293 cells (as well as other cell cultures) as expression hosts for suspension culture. Milligram to gram levels of recombinant protein/antibody can be produced by the system. At present, our products have been widely used in antibody production, disease diagnosis, vaccine development and other fields.
Target gene is transferred into specific mammalian cells to express the target protein. In the transient transfection system, the exogenous gene is always present in the free vector rather than integrated into the cell chromosome. This way, gene expression products can be obtained in a short time, but with the continuous division and proliferation of cells, foreign genes will be lost, and recombinant protein production cannot continue. The transient expression system has the advantages of short expression time, simple steps and short experimental period. Large-scale transient expression technology is a research hotspot in recent years.
Currently, Creative BioMart provides the following transient mammalian cell expression services based on different expression vectors.
See Features and Comparison of Virus and Plasmid-Mediated Mammalian Expression Systems→
Creative BioMart's transient mammalian cell expression services outline is listed below. For customers who are interested in our services please feel free to contact us or make online inquiry.
| Service Name | Service Description | Timeline |
| Pre-service consultation | Understand protein expression requirements, provide alternative solutions | —— |
| Expression System Selection | Expression vector: Creative BioMart's featured vectors / conventional commercial vector Host cells: a variety of cell lines in serum-free medium Transfection reagent: matching the system |
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| Transient Vector Construction | Gene optimization and design, gene synthesis and subcloning construction | 2-4 weeks |
| Expression and Purification | Endotoxin-free Plasmid Extraction；plasmid quality control and transient transfection； expression conditions optimization; SDS-PAGE / WB analysis and identification； optimal expression conditions expansion and purification test；label removal test | 2-3 weeks |
| 1-10 L Expression & Purification | Endotoxin-free Plasmid Extraction; plasmid quality control; transient transfection; protein affinity purification (or other purification methods); protein plasmid identification; MS identification; label removal | 2-4 weeks |
| Scale Up Service | Endotoxin-free Plasmid Extraction; plasmid quality control; transient transfection; protein affinity purification (or other purification methods); protein plasmid identification; MS identification; label removal | 10-20 L: 3-5 weeks 20-50 L: 4-7 weeks More than 50 L: 5-12 weeks |
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMarts' protein expression services, please feel free to inquire about more details.
