T7 Regulated E. coli Extract Protein Synthesis Service

For customers who are looking for a high-yield E. coli cell-free protein synthesis, please consider Creative BioMart's T7 Regulated E. coli Extract Protein Synthesis System. It only takes up to 2 hours to express the target protein from the reagent mixture and the yield can reach around 0.5 mmg/ml./p>

Technology Overview

The system consists of:

• Highly active E. coli cell extracts that are prepared by using the mutant E. coli B with deficient OmpT endoproteinase and lon protease activity.
• Optimized T7 RNA polymerase that promotes efficient transcription
• RNase inhibitors that inhibit potential RNase contamination carried over from plasmid preparation
• Protein synthesis reaction buffers containing energy sources, nucleotides and amino acids

Service Description

The TEMPLATE is the only thing that needs to be provided by the client. Here are some corresponding suggestions that you may need when designing your template.

A. The template can be plasmid DNA, linear DNA or mRNA. Plasmid DNA usually shows a higher yield.

Please make sure your template meets all the above requirements before sending it to us. In addition, we welcome your authorization to let us design and synthesize target sequences for you.

Technology Features and Applications

The main features of this technology are as follows:

• High yield. Using T7 promoter, the protein yield can reach 0.5 mg/ml
• It's fast. Protein can be produced in 2 hours and the process is visible
• Flexibility. The template can be plasmid DNA, linear DNA or mRNA
• Low background, low level of endogenous protein synthesized by the system.
• It can synthesize 17 to 230 kDa target proteins
• Adding RNase inhibitor to the reaction system can inhibit RNase pollution
• Proteins can be separated by affinity purification or functional analysis directly
• Protein synthesis can last more than 8 hours at 37°C

The system can be applied to the synthesis of radioisotope-labeled proteins, verification of cloned genes before protein expression in E.coli, functional study of protein transcription and translation, protein as a tracer for protein purification, and structural study of protein by mixing non-natural amino acids.

Related services

In addition to the high yield protein expression service above, we also provide Classic E. coli Extract Protein Synthesis Service. In the system, the template can be provided in the form of plasmid DNA, Liner DNA or mRNA.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.