E. coli is one of the most widely used hosts for producing heterologous proteins. It has excellent genetic characteristics and the advantages of rapid propagation, easy cultivation and low price. Creative BioMart has more than 10 years of development and research experience in the field of recombinant protein production. Just submit the coding sequence of the target protein, Creative BioMart’s professional technical team will not only carry out efficient one-step protein expression, but also provide optional services (like expression condition test, endotoxin removal, protein labeling, etc.) to optimize the expression integration.
A variety of optional services —— Optional services are provided to improve the expression
Professional consultation service —— Provide consulting before and after the submission of the target sequence, aiming to identify the specific requirement and design the best expression strategy.
Customized service —— We are able to provide customized service for customers who have special expression requirement.
Professional ethics —— Strictly abide by trade secrets, we strictly keep confidential the information provided by customers.
Guaranteed success rate —— The delivery rate of bacterial expression service of Creative BioMart is as high as 99%.
Production procedure outline
| Service Name | Service Description | Deliverable |
| Expression strategy design | Expression strategy designed referring to customers’ requirement and protein properties |
|
| Codon optimization and gene synthesis (2 weeks) | Optimize the codon in order to improve protein yield significantly | |
| Protein expression, evaluation and optimization (1 week) | We will conduct a series of pilot expression tests using diverse expression conditions and take SDS-PAGE and western blot to confirm the expression levels. At this step, we will optimize your protein expression conditions. | |
| Scale-up and protein purification (2-3 weeks) | We will scale up your protein expression using optimized conditions. After multistep protein purification, the protein purity will be >90%, and endotoxin level will be <1 EU/μg. |
Basic Principle of E. coli Expression System
Expression pathways
Creative BioMart’s bacterial expression platform supports operon regulated E. Coli expression pathways including
Comparison Among Different E. Coli Expression Pathways→
We also offer special form protein expression services for customers who need to produce inclusion body, secretory protein, fusion protein, oligomeric protein and integrated protein.
Optional services
Based on our customized solutions and rich experience, Creative BioMart offers the following optional services:
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.
