Stable Polyclonal Drosophila S2 Cell Line Construction Service

Schneider-2 (S2) Drosophila cell line is very suitable for stable overexpression of recombinant protein using a plasmid-based protein expression vector. Compared with the transient bbaculovirus-assisted overexpression of the recombinant protein in insect Sf 9 cells, S2 cells can be used to stably express a variety of recombinant protein cells using vectors specially designed for protein expression in Drosophila.

Stable polyclonal Drosophila S2 Cell Line Construction Service

Stable polyclonal Drosophila S2 cell line construction service is now available at Creative BioMart. Both the pre-construction and post-construction steps like codon analysis, optimization, cell line productivity test are all included in our one-stop service.

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Here we describe the basic protocol of effectively transfecting plasmid DNA into S2 cells of Drosophila to stabilize overexpression of target protein. The specific presentation process may be changed according to your requirements.

We can transfer plasmid DNA into S2 cells calcium phosphate precipitation transformation.

Service Name	Service Description
Gene synthesis and subclone	Protein gene sequence analysis. Codon optimization and gene synthesis Vector construction and plasmid preparation
Host cell line transfection	S2 cell culture preparation S2 Cell line transfection Expression test (WB or ELISA) Positive clone screening
Stable cell line construction	Stable cell culture screen with stable drug pressure for around 4 weeks Protein production Freeze cells for storage (short-term or long-term )
Deliverable	Expression vector Sequencing report Stable cell culture (1x 107 cells/ml)

Features of S2 Expression System

In addition to all the advantages of the Sf9 cell system, the S2 system has additional advantages.

The culture condition is simple. The S2 cell line can be easily maintained at room temperature without the need for an incubator with CO2 supply.
S2 cells can grow abundantly in the medium, and they also have an effective silencing mechanism (RNAi).
The S2 cell system supports the constitutive, inducible and transient expression of proteins.
The inducible vector of the system allows the regulation of gene expression and can be used to produce a large number of proteins for protein crystallography research.
The S2 cell genome can be modified by a binary expression system.

Related Services

In addition to the stable cell line construction service, we also offer the following related services:

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

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