Kluyveromyces Lactis has been successfully applied to the expression and production of many heterogeneous proteins. Compared with other yeast, it has many advantages, such as super secretion ability, satisfying characteristics in large-scale fermentation, food safety level and integrated expression ability, etc. It has also shown great potential as a host system to express medicinal proteins. Protein expression service based on the Kluyveromyces Lactis is now available at Creative BioMart.
Compared with other yeast, K. lactis has many advantages. Here is the comparison of several commonly used yeast expression systems.
| Yeast Species | S. cerevisiae | K. lactis | Y. lipolitica | P. pastoris | H. polymorpha |
| Production of industrial enzyme | Yes | Yes | Yes | Not Available | Not Available |
| Food safety | GRAS | GRAS | Non-GRAS | Non-GRAS | Non-GRAS |
| High-density fermentation | Yes/ No | Yes | Yes | Yes | Yes |
| Fermentation explosion | No | No | Explosive | Explosive | Explosive |
| Secretion | No | Yes | Yes | Yes | Yes |
| Hyper-glycosylation | Yes | No | No | No | No |
| Genetic manipulation | Easy | Easy | Not easy | Not easy | Not easy |
| Episomal vectors | Stable | Stable | Not stable | Not stable | Not stable |
Figure 1. K. lactis expression system (An emerging tool in biotechnology, 2016)
We offer basic choices for two types of expression systems.
The gene of interest can be expressed through episomal vectors. This system is less stable than the integrated ones, but it is suitable for rapid protein expression. Additionally, this system is competent for secretory protein expression.
The integrated vector contains some homologous regions of Kluyveromyces lactis, thus the vector can be inserted into the genome by homologous recombination. The expression system has high genetic stability and can be used for large-scale fermentation, but the construction time of the expression system is longer than the episomal system.
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