The major limitations of using hybridism technology to produce monoclonal antibodies include the quality differences between antibody batches, the risk of loss of antibody specificity, and the immune response caused by animal-derived antibodies. One effective solution to these limitations is to sequence and express the antibody through recombination mammalian protein expression.
With decades of development, Creative BioMart has gained extensive experience in the field of recombination protein expression. Our service scope covers full-length antibodies, scFv, Fab fragments, single domain antibodies, nanobodies, chimeric antibodies, and diverse Fc fusion proteins.
In order to achieve reproducible, rapid, stable, and large-scale production of monoclonal antibodies, the monoclonal antibodies will usually be sequenced at the gene level. The corresponding sequences of the antibodies will then be connected to the expression vector, and finally expressed using a recombination protein expression system.
The recombination monoclonal antibody has the following advantages:
Recombinant antibody production needs 6-8 weeks, while hybridoma antibody preparation is generally more than 4 months.
The process of recombination antibody production does not involve the immune response process, avoids allergic reactions caused by animal-derived antibodies. Additionally, no animal welfare issues are involved.
The recombination antibody gene is confirmed and the expression is stable.
No antibody specificity will be lost due to cell freezing or long-term cell culture
The antibody can be modified during the construction of recombination vectors, such as antibody fragment expression, isotype replacement, chimeric antibody, antibody humanization, etc.
Recombinant antibody expression
Customers only need to submit the antibody gene sequence online, and we will be able to provide you with various forms of recombination antibody production services according to your requirements. Creative BioMart’s technology platform also provides a monoclonal antibody sequencing service based on RACE(Rapid Amplification of cDNA Ends Technology).
| Customers provides | Service procedure | Timeline | Deliverable |
| Antibody DNA sequence/ hybridoma / antibody sample | hybridoma / antibody sample sequencing (optional) | 2 weeks |
Inquiry |
| Target DNA synthesis & vector construction | 1-2 weeks | ||
| Plasmid extraction | 3-4 weeks | ||
| Transient transfection & and antibody expression | |||
| Antibody purification | |||
| Antibody quality test |
Our platform can complete high-throughput gene synthesis. If you need to prepare antibodies for high-throughput antibody screening, please visit our high-throughput recombination antibody one-stop service page.
High-affinity antibodies screening
Creative BioMart can also help customers with high-affinity antibodies screening. Just provide us with antigens or antigen sequences, we will be able to conduct the screening through phage display technology.
By constructing a phage antibody library, antibodies with high affinity can be screened and expressed. The basic process is shown in the figure below:
Creative BioMart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.
