Lactococcus lactis is a homotype fermentation bacteria, which can realize the rapid transformation of lactose to lactic acid. The functional characteristics of Lactococcus, such as carbon metabolism, extracellular and intracellular proteolysis system, antibiotic production, phage interaction and resistance, have been widely studied, which promote the wide application of Lactococcus in biotechnology.
Creative BioMart's has introduced the perfect expression system of lactic acid bacteria, construct mature vectors, and provide our customers with high-quality customized service of recombinant protein expression. Our biological protein expression core technology and resource advantages have determined our outstanding service quality.
In recent years, Lactococcus lactis has been identified as an effective Gram-positive cell factory for the production of recombinant protein. According to the types of promoters in the genome, the expression system of Lactococcus can be divided into constitutive expression system and inducible expression system. Expression services based on these two systems can be found in our technology platform. Please click the following link to learn more about the service details.
Constitutive Lactococcus Expression Service
Traditional Lactococcus expression pathway. The process of foreign gene expression is not affected by the expression period, expression site or environment. The level of gene expression is constant. Mass production of various proteins has been realized by this system. The system can keep high output continuously. According to the requirements of customers, we can provide food-grade and non-food-grade expression channels
Inducible Lactococcus Expression Service
Regulatory expression system. Lactose, sucrose, nisin, purine and other edible substances can be used as inducers to produce food grade products. Currently, the most famous inducer is nisin, but we can also replace it with other inducers according to the needs of customers.
Please contact us if you need the following services:
The basic framework of our expression service is as follows, and the specific scheme will be slightly changed according to the requirements of customers
1. The choice of expression system
2. Construction of expression vector
3. Protein expression test and identification
4. Protein expression and purification
5. Scale-up culture in fermentor
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart protein expression services, please feel free to inquire about more details.
