Figure 1. Secretion pathway of yeast (Cellobiohydrolase secretion by yeast: Current state and prospects for improvement, 2013)
Yeast expression system is the most economical and efficient eukaryotic protein expression system, which can achieve intracellular expression or secretion expression successfully. Moreover, its amplification medium is relatively cheap, and the requirements of culture conditions are not high, so it is suitable for industrial amplification. Like mammalian cell expression system, yeast protein expression system can modify the expressed protein to ensure its natural conformation, such as glycosylation, acylation, lipoylation and phosphorylation, which can be used to prepare high value-added protein materials that are very close to the natural protein.
After years of development, Creative BioMart has established a complete yeast expression technology platform. Currently, we are able to offer our clients the expression services based on three yeast species.
Pichia pastoris is a methylotrophic yeast. It provides an effective platform for the production of foreign proteins. Platform components are available as kits and have been widely used in protein production worldwide in academia. Strains that can produce complex human N-glycans have been designed.
Saccharomyces cerevisiae is a traditional Baker's yeast, which is widely used in brewing and baking. Yeast is generally referred to as a single species. As an expression platform, it has been successfully used in the production of enzymes, insulin and hepatitis B vaccine.
Yersinia lipolytica is a bimodal yeast, which can grow on a variety of substrates. Therefore, it has great potential for industrial application.
Based on the yeast species above, our services include but are not limited to
For detailed information, please turn to our Service page.
In addition to a one-stop heterologous protein expression service, we also provide a wide range of products. In our product library, you can find the products needed in all expression pathways, such as expression kits, strains, media, vectors, transfection reagents, etc. In addition, our scientific consultants can provide professional consultation services so that customers can complete high-quality protein expressions in their own laboratory.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
Reference
