The use of recombinant algae for the production of valuable compounds has triggered promising biotechnology applications. In the microalgae expression system, some viral components have been used for innovative genetic engineering methods. Marine microalga Schizochytrium SP is an attractive species that can heterotrophically grow in seawater rather than depend on photobioreactors.
Creative BioMart has developed an inducible viral vector-mediated Schizochytrium SP expression system which allows for a fast and efficient recombinant expression, overcoming the unsolved low yields and unstable expression patterns frequently observed in the conventional microalgae expression systems.
To construct the system, an inducible viral vector is designed to harbor AlcA promoter from Aspergillus nidulans. The Rep replication protein is added to allow the expression cassette to be expressed at high levels. The detailed vector map is shown as follows.
The subclone and amplification process needs the participation of Agrobacterium tumefaciens. After PCR confirmation, the recombinant expression vector will be transformed into the Schizochytrium sp strain by inoculating with A. tumefaciens culture.
20 hours after inoculation, the target gene will be expressed under the induction of anhydrous ethanol, and reach the maximum yield at 48 hours.
| Service Name | Service Description |
| Expression strategy design | Client provides the target sequence and expression strategy designed referring to customers’ requirements and protein properties |
| Codon optimization and gene synthesis |
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| Construction of the expression cell strain |
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| Protein expression, evaluation and optimization | Induce expression with anhydrous ethanol, the yield may peak at 48 hours post-transformation |
| Deliverable |
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