Creative BioMart's piggyBac vector system is highly effective for inserting foreign DNA into the host genome of mammalian cells. This system is technically simple, utilizing plasmid transfection (rather than viral transduction) to permanently integrate your gene(s) of interest into the host genome.
Our piggyBac plasmid along with the helper PBase plasmid are optimized for high copy number replication in E. coli, efficient transfection into a wide range of target cells, and high-level expression of the transgene carried on the vector.
Conventional transfection results in almost entirely transient delivery of DNA into host cells due to the loss of DNA over time. This problem is especially prominent in rapidly dividing cells. In contrast, transfection of mammalian cells with the piggyBac transposon plasmid along with the helper plasmid can deliver genes carried on the transposon permanently into host cells due to the integration of the transposon into the host genome.
Delivering plasmid vectors into cells by conventional transfection is technically straightforward, and far easier than virus-based vectors which require the packaging of live virus.
Our transposon vector can accommodate ~30 kb of total DNA. The plasmid backbone and transposon-related sequences only occupy about 3 kb, leaving plenty of room to accommodate the user's sequence of interest.
The system is derived from the piggyBac transposon, which is originally isolated from the cabbage looper (Trichoplusia ni, a moth species). Based on sequence homology, the piggyBac transposon was found to belong to a class of transposons common to many animals.
The piggyBac system contains two vectors, both engineered as E. coli plasmids.
The gene to be delivered into host cells is cloned into this region.
When the PBase vector and the piggyBac transposon vector are co-transfected into target cells, the transposase produced from the helper would recognize the two TRs on the transposon, and insert the flanked region including the two TRs into the host genome. Insertion typically occurs at host chromosomal sites that contain the TTAA sequence, which is duplicated on the two flanks of the integrated fragment.
Creative BioMart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.
