As a mono cytoplasmic eukaryote, yeast has a relatively complete gene expression regulation mechanism and the ability to process and modify the expression products. Pichia pastoris is a kind of methylotrophic yeast. It is one of the most effective expression systems of exogenous protein in recent years. At present, many foreign proteins have been successfully expressed in the host system.
As an expert in protein expression, Creative BioMart has established a multi-functional yeast expression platform including a variety of strains and expression vectors. Based on this platform, we are able to provide classic expression services for intracellular and secretory proteins for our clients.
Sines there is no stable additional plasmid in Pichia pastoris, the expression vector needs to be homologous recombination with the host chromosome, and the foreign gene expression framework is integrated into the chromosome to realize the expression of foreign genes. The advantage of integrated expression is to maintain the stability of foreign genes and produce multiple genes.
The classic Pichia pastoris expression vector we offer contains the regulatory sequence of alcohol oxidase gene. The main structures include:
Intracellular expression system
If your protein is cytosolic and non-glycosylated, you can choose to express the protein intracellularly. We offer three corresponding vectors (1) pAO815; (2) pPIC3K (3) pPUC3.5K, as well as two host strains (1) KM71; (2) GS115 for clients to choose from.
| Expression Type | Vector Name | Screening Mark | Promoter |
| Intracellular Expression | pAO815 | HIS4 | AOX1 |
| pPIC3K | HIS4 | ||
| pPUC3.5K | HIS4 | ||
| Secretory Expression | pPICZα,A,B,C | Bler | |
| pPIC9K | HIS4 | ||
| GS115 Strain: AOX1 and AOX2 genes express AOX protein (ethanol oxidase) normally, and the utilization rate of methanol is high, which is close to the wild type and belongs to the Mut+ | |||
| KM71 Strain: AOX1 gene is mostly deleted, and only AOX2 could be used to encode AOX. The utilization rate of methanol becomes slower, which shows that it is Muts | |||
Secretory expression system
Pichia pastoris has very low levels of secretory native proteins. Therefore, the secreted heterogeneous protein comprises the vast majority of the total protein in the Pichia growth medium. For the secretory expression, we offer two corresponding vectors (1) pPICZα,A,B,C; (2) pPIC9K, as well as two host strains (1) KM71; (2) GS115 for clients to choose from.
In addition to the signal peptide from the expression vector, the following signal peptide can also be selected.
| Signal Peptide | Protein Sequence |
| αMF | MRFPS IFTAV LFAAS SALAA PVNTT TEDET AQIPA EAVIG YSDLE GDFDV AVLPF SNSTN NGLLF INTTI ASIAA KEEGV SLEKR EAEA |
| Dse4 | MSFSS NVPQL FLLLV LLTNI VSG |
| Exg1 | MNLYL ITLLF ASLCS A |
| Scw11 | MLSTI LNIFI LLLFI QASLQ |
| Salb | MKWVT FISLL FLFSS AYS |
Did not find what you need? In addition to the classic expression services for both intracellular and secretory proteins, we also offer
Reference
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
