In Creative BioMart's yeast expression platform, clients can find the surface display service mediated by agglutinin. The inner layer of the Saccharomyces cerevisiae cell wall is mainly composed of dextran skeleton, which contains GPI (glycosylphosphatidylinositol) anchored regions. By fusing the heterologous target protein gene sequence with the cell wall protein sequence (like agglutinin and flocculin Flo1, Sed1, Cwp1, Cwp2, Tip1, and Tir1/Srp1) and introducing it into yeast cells, the target protein can be localized and expressed on the yeast cell surface by the mechanism of protein transport to the membrane surface (GPI anchoring). a-agglutinin and α- agglutinin are two kinds of mannose proteins in the yeast cell wall. We will provide yeast surface display services by fusing foreign proteins with the two kinds of agglutinin sequences.
The C-terminal of α-agglutinin contains GPI anchored signal sequence, so it can be anchored on the glucan of the yeast cell wall. To start the display program, we will
A-agglutinin consists of core subunit (Aga1p) and binding subunit (Aga2p). There are 725 amino acids in Aga1p, which are covalently linked with β-glucan in the yeast cell wall. There are 69 amino acids in Aga2p, which bind to Aga1p through two disulfide bonds. After the fusion expression of C-terminal and N-terminal of Aga2p subunit, the target protein can be displayed on the cell surface by covalent connection of Aga1p and yeast cell wall.
Did not find what you need? We also offer
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
