Membrane protein constitutes more than half of the targets of commercial drug treatment in the world market, so the development of membrane protein expression technology has become a hot spot. Like many water-soluble proteins, membrane proteins can be produced from a variety of common cell expression systems. system will cause a series of problems due to overexpression of heterologous proteins, such as insufficient membrane insertion, aggregation and degradation of target membrane proteins, which greatly hinder the application of cell-based protein expression.
The above problems can be solved by using the cell-free expression system. Creative BioMart now offers our global customers high-quality custom services to express the membrane protein with cell-free protein synthesis system (CFPS).
At Creative BioMart, we are able to synthesis G protein-coupled receptor (GPCR), liposome, micelles and nanodiscs with E.coli CFPS, Rabbit Reticulocyte Lysate CFPS, Wheat Grem Extract CFPS, Insect CFPS, Mammalian CFPS, PURE CFPS, and Yeast CFPS.
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Compared with cell-based methods, innovative cell-free machines offer many advantages, such as stable expression, small reaction volume, easy purification and no need for detergent. Proteins synthesized from scratch can be directly reconstituted into various forms, including detergents, liposomes or nanodisks. Prokaryotic (E.coli) and eukaryotic (wheat germ, rabbit reticulocyte lysate, insect cell lysate, CHO / HEK lysate) are available, from which many GPCRs have been successfully obtained at the level of mg.
We can now provide high-quality customized services for global customers to express cell-free membrane proteins into liposomes.
Screening the best liposomes may be laborious, time-consuming and expensive. We use more than 30 kinds of bilayer lipids and various bilayer lipid additives in different studies. No detergent will be used at any step. We can test the effects of different lipid bilayer additives on membrane protein folding, conformation, oligomerization, function and activity according to customers' requirements.
Solubilization and stability medium is one of the key factors to determine the expression quality of the non-cell membrane protein. In our technology platform, more than 100 kinds of detergents and lipids can be used alone or in combination to form micelles. Based on our powerful detergent screening platform and lipid screening platform, our experienced researchers can always find the most suitable choice to meet each specific customization requirement.
As one of the best biomembrane mimicry systems, nanodisks are widely used for the solubilization and stabilization of membrane proteins. The membrane proteins incorporated into the nanodisks can be used for immunity, drug and antibody screening, protein-protein interaction studies using SPR and BLI, and structural studies using NMR and cryo-EM. We have rich experience in the expression of membrane proteins and the preparation of nanodisks. We will use bilayer lipid and membrane scaffold protein (MSP) to directly incorporate the newly synthesized membrane protein into the pre-assemble nanodisk.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
