Retrovirus-Mediated Stable Mammalian Cell Line Establish Service

The retroviral expression system is a new highly efficient expression system of recombinant protein which can mediate the stable and long-term expression of foreign genes. It has been widely used in foreign gene expression, gene silencing, gene therapy, and biopharmaceuticals because of its simple genome structure, easy gene manipulation, high transfection efficiency, wide host range and easy separation.

The expression system is composed of the retroviral expression vector, auxiliary vector (expressing protein needed for viral packaging) and packaging cell line.

Creative BioMart's retroviral expression system mainly uses the moloni mouse leukemia virus (MMLV). The plasmid backbone has a variety of optional screening markers for customers to choose from. Plat-E cells are used as packaging cells so that the plasmid carrying exogenous gene can be packaged by simply being transferred into the packing cell, which is more convenient to operate.

Why Choose Retrovirus Expression System

• The expression system can effectively mediate the stable and long-term expression of the target gene.
• High transfection rate, so the target gene is more likely to be integrated into the host cell genome.
• The expression system can be used to overexpress genes and induce the production of pluripotent stem cell lines
• The rapid establishment of stable expression cell lines can shorten the experimental period and improve efficiency.

Basic Service Procedure

A. Construction of retroviral vector: the target gene fragment is connected to the retroviral vector.

B. Retroviral packaging: the recombinant virus plasmid is directly transfected into Plat-E cells for viral packaging. The cell supernatant rich in retrovirus particles is collected. The titer of retrovirus should at least reach 1 × 10⁶ ~ 1 × 10⁷ Tu/ml.

C. Establishment of stable cell lines: the experimental group and the control group are used to infect the cells with retrovirus, and the stable cell lines are preliminarily screened by antibiotics, and the monoclonal cell lines are obtained by cell sorting.

Deliverable

At the end of the experiment, Creative BioMart will submit complete experimental results to our customers including:

Creative BioMart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.