Mammalian cells have the ability to express foreign recombinant proteins that is close to the human conformation, so it has become the preferred host cells for the production of a variety of biological medical proteins. For stable mammalian cell expression system, it is essential to ensure high-efficiency protein production to satisfy industrial demand. Creative BioMart provides several expression optimization solutions to customers in need, from high-efficiency expression vector construction, host cell modification, high-throughput screening, to medium optimization.
Establishing a high-efficiency expression gene carrier is known as the main access to improve protein production level. The major objective of vector construction is either to increase the foreign gene ratio or increase the stability of the host cell line. Creative BioMart applies the following strategies to achieve the goal for customers.
The expression level of recombinant protein depends on the integrated viable living cell density (IVCD) and unit cell productivity. In order to improve IVCD, Creative BioMart provides host cell modification solutions focusing on anti-apoptosis, accelerating proliferation, increasing cell growth rate and increasing maximum activity density. To improve the unit cell productivity, our manipulation plan usually starts from protein folding, transportation, modification, to secretion.
In order to deal with the uneven clone productivity caused by the expression process, Creative BioMart recommends fluorescence-activated cell sorting (FACS) technology to assist the efficient screening of high-yield positive clones. Our platform supports four screening strategies based on FACS. According to different needs, customers can choose to
(1) Introduce fluorescent protein genes directly during the construction of vectors;
(2) Labeling cells with gel droplet method;
(3) Labeling cells with affinity matrix adsorption;
(4) Cold trap method for fluorescent labeling.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart’s’ protein expression services, please feel free to inquire about more details.
