For clients who need to carry out routine protein synthesis without urgent delivery of products, we recommend you to choose Creative BioMart's Classic WGE Protein Synthesis Service. This service is economical and less time-consuming, which can fully meet your needs.
We have established an economical WGE system, which can realize small/medium/large-scale protein expression through linear template or plasmid template. In this system, transcription and translation are uncoupled. Firstly, the template DNA is transcribed, and then mixed with a wheat germ cell-free protein expression system to complete mRNA translation (the mix contains all the necessary enzymes, various tRNAs and ions for protein translation), so as to obtain the target protein. We used a bilayer liquid reaction (bilayer) to express the target protein.
We usually use a series of plasmid PEU as the expression vector, and clone the cDNA with start codon and stop codon into the vector, then extract the plasmid and use it directly for protein expression. We can also use other expression vectors with SP6 promoter for protein expression.
Bilayer reaction method is used for transcriptionreaction occurs at the intersection of the liquid surface. As time goes on, the solution gradually changes from two phases to a single mixture.
The specific information of different reaction systems is as follows:
| Reaction Scale | Time | Expression Level | Price |
| Small | 1-4 hours | 1-3 μg | Inquiry |
| Medium | 4 hours | 30 μg | Inquiry |
| Large | 1 day | 0.5-4 mg | Inquiry |
The expressed product can be used in Western blot, CBB staining and other protein detection experiments, protein structure analysis, immunoprecipitation and other common protein function analysis.
Didn't find the service you need?
If you need to express the membrane protein, please choose WEG Membrane Protein Synthesis Service.
If you have requirements for the synthesis period, we recommend High-Yield WGE Protein Synthesis Service, which can synthesize your target protein within 2 hours.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
