Large-scale protein expression is a prerequisite for scientific research, but there are still some difficulties. Creative BioMart has a mature protein expression system and advanced protein expression technology.
Our large-scale preparation services include: protein fermentation, high-density fermentation technology and process optimization. We have many types of fermenters, bioreactors and protein purification systems. We offer recombinant proteins from mg to g quantities. Customers can choose the appropriate expression system and fermentation scheme according to their own needs.
As an expert in biotechnology, Creative BioMart has established a complete fermentation system. We have different sizes of fermentor, professional equipment and professional technicians. We promise to provide clients with customized expression designs and high-quality products.
The following is the basic process of large-scale fermentation service. The specific steps can be changed according to the different protein properties, and everything will be subject to the formulated expression designs.
| OUR SERVICES | FERMENTATION QUANTITY | DELIVERABLE | TIMELINE |
| Protein fermentation | 5-50 mg/L |
|
1 week |
| High-density fermentation | 20-400 mg/L | 3 weeks | |
| Process optimization | 100-1000 mg/L |
|
8-12 weeks |
Gene optimization
Gene sequence is the key factor for protein expression. We use bioinformatics software to provide customers with optimization items, including mRNA structure, rare codons, ribosome binding sites, etc
Optimization of expression conditions
The factors affecting protein expression, including temperature, inducer and OD, are optimized to obtain higher cell density and protein expression in non-fermentation conditions.
Large-scale preparation
Classic protein fermentation and high-density fermentation.
Protein purification
To obtain a large number of protein expression bacteria mud, we can provide a variety of protein purification methods, and ultimately deliver proteins with the purity required by customers.
We adopt fed batch strategy to ensure a high yield of high-density protein fermentation. By adjusting the culture conditions, temperature and pH value, and the feeding rate of culture and substrate, cell quality and productivity can be improved to the maximum.
Our fed batch strategy
In order to achieve an exponential growth rate
Control oxygen, pH, CO2 release rate. The cell concentration is measured by laser turbidimeter to determine the feeding speed.
The feeding rate is determined by the concentration of carbon source in the fermentation process.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.
