Baculovirus-Mediated Protein Expression Service

Baculovirus is a kind of virus that takes the arthropod as its single host. Studies have shown that wild baculovirus can enter mammalian cells without causing any cytopathic effects. Consequently, the insect virus can be applied as an expression vector to mediate the protein expression within mammalian cells. The novel system is named “BacMam expression system” and it has been widely used in various fields of biotechnology and medical treatment.

Service Description

As an expert in the field of biotechnology, Creative BioMart provides customers with comprehensive construction methods of BacMam for customers to choose according to their needs.

In short, BacMam is the combination of transient mammalian expression system and insect cell expression system. The target genes are assembled into particles which can be recognized by mammalian cells in insect cells, and then enter mammalian cells through endocytosis to mediate the synthesis of foreign proteins.

Presently, Creative Biomart's mammalian cell platform supports three different BacMam versions, each has its own features. For customer who are interested in our BacMam services, please click the links below to get access to more detailed information.

By choosing BacMam service, you can be benefited from the superior features in the following aspects:

• Safe – Low biological hazard, does not replicate in mammalian cells and has no cytopathic effect
• Low toxicity – Ability to reduce overt cytotoxicity
• Efficient – Has high transduction efficiency for mammalian cell lines, primary cell lines and stem cells
• Wide host range – Can be transferred into many mammalian cell lines
• Regulatory – Gene expression can be easily regulated by changing the amount of transduction

Recommended Application

• Rapid – Expression is much faster than the generation of stable cell lines

Typically our customers will adopt the BacMam services

• Deliver vectors for genes in mammalian cells.

Including nuclear receptors, secreted proteins and membrane proteins, such as G protein-coupled receptors (GPCR), drug transporters and ion channels.

• Functional characterization and protein production for structural studies
• Cell membranes production
• High content screening assay development
• Vaccine production
• Toxic proteins production

If you have any questions or requests, please feel free to contact us or make online inquiry. Our customer service is always ready for you 24/7.