Ecdysone is an insect steroid hormone that can trigger metamorphosis in insects. Currently, ecdysone-regulated gene switches have been developed into inducible expression systems for mammalian cells. The low basic activity and high induction rate of the mammalian expression system induced by ecdysone make it an attractive alternative to strictly regulate gene expression in cultured cells and transgenic mice.
The ecdysone-inducible mammalian expression system is now available at Creative BioMart. We provide services for inducible expression system construction and target protein production based on the system.
The ecdysone-inducible expression is consists of a Mammalian cell strain which harbors both the gene for the Drosophila melanogaster ecdysone receptor and a promoter containing a binding site for the ecdysone receptor. The transcription can be activated by insect hormone ecdysone or its analog ponasterone A (ponA).
Receptor plasmid 1 can express VgEcR and RXR proteins constitutively.
Expression plasmid 2 contains a ponA-inducible expression cassette (E/GRE recognition sequence), MCS for foreign gene insertion.
In the absence of ponA induction, the promoter is tightly inhibited by covasopressin. When ponA binds to VgEcR, the corepressors are released, coactivators are recruited, and the complex becomes transcriptionally active.
Here is the protocol we use to establish the ponA-inducible expression system for clients.
| Service Name | Service Description |
| Expression strategy design | Client provides the target sequence and expression strategy designed reflecting customers' requirements and protein properties |
| Construction of the expression vector |
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| Establishment of the stable cell culture of receptor plasmid 1 |
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| Inducible protein expression |
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| Deliverable |
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Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
