Economic Crop Transgenic Operation

Economic crops, also known as industrial raw material crops, generally refer to all crops with specific economic value. Creative BioMart provides standardized transgenic operation services for scientific research institutions and provides technical support for the field of genetics and breeding. Our transgenic plants mainly include cotton, soybean, cucumber, tomato and rapeseed. Please note that the service is only for experimental research, not for actual production.

Cotton (Anemone vitifolia Buch ) Transgenic Service

Figure 1. Flow chart for obtaining transgenic cotton via Agrobacterium-mediated transformation (Zhang, 2013)

The experimental period is 4-5 months.

Service Content

T1 generation seed cultivation, including at least 20 positive strains Screening pure lines

Provided Results

Progress description. Vector construction, sequencing report and images. Antibody screening, plant transplanting and other images. The target gene PCR of T1 resistant plants is detected by gel electrophoresis.

Soybean (Glycine max) Transgenic Service

The experiment will last about 4-6 months. The transgenic process is similar to that of the cotton strain and the provided results will include

• Progress description.
• Vector construction, sequencing report and images.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• The target gene PCR of T0 generation regenerated plants is detected by gel electrophoresis.

Figure 2. Depiction of transformation, selection, and regeneration of half-seed explants of Glycine max (Hada, 2018)

Cucumber (Cucumis sativus L.) Transgenic Service

4-6 months required. The transgenic process is similar to that of the cotton strain and the provided results will include:

• Progress description.
• Vector construction, sequencing report and images.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• Target genes PCR of T0 generation are detected by gel electrophoresis or GUS.

Tomato (Lycopersicon esculentum) Transgenic Service

Figure 3. Hygromycin resistant shoots regenerated on selective media (Saker, 2011)

4-7 months required.

• Bottle seedlings of T0 generation positive plants (2-4 plants)
• Bottle seedlings of T0 generation positive plants (10 plants)
• Bottle seedling cultivation and transplanting

The transgenic process is similar to that of rice cotton and the provided results will include:

• Progress description.
• Vector construction, sequencing report and images.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• The target gene PCR of T0 generation regenerated plants is detected by gel electrophoresis.

Rape (Brassica napus L.) Transgenic Service

4-6 months required. The transgenic process is similar to that of the cotton strain and the provided results will include:

• Progress description.
• Vector construction, sequencing report and images.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• The target gene PCR of T0 generation regenerated plants is detected by gel electrophoresis.

For all the services above, customers can directly provide the constructed transformation vector, or entrust our company to construct various vectors such as gene cloning, expression and knockout. We will complete the whole experiment process from the construction vector to the acquisition of transgenic seedlings for you.

The Overview of the Biological Service Process

• Sign service contract;
• The client transfers the target gene and makes the payment;
• We carried out transgenic experiments;
• We deliver the experimental results to the customer, and the customer checks and accepts them.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

References

1. Zhang B. Transgenic cotton: from biotransformation methods to agricultural application. Methods Mol Biol. 2013;958:3-15.
2. Hada, A., Krishnan, V., Jaabir, M.S., Kumari, A., Jolly, M., Praveen, S., & Sachdev, A. (2018). Improved Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merr.] following optimization of culture conditions and mechanical techniques. In Vitro Cellular & Developmental Biology - Plant, 54, 672-688.
3. Saker, M. , Salama, H. , Salama, M. , El-Banna, A. , & Abdel Ghany, N. (2011). Production of transgenic tomato plants expressing Cry 2Ab gene for the control of some lepidopterous insects endemic in Egypt. Journal of Genetic Engineering and Biotechnology, 9 (2).